Escherichia coli lipopolysaccharide administration transiently suppresses luteal structure and function in diestrous cows.

摘要: The objective was to characterize the effects of Escherichia coli lipopolysaccharide (LPS) endotoxin (given i.v.) on luteal structure and function. Seven nonlactating German Holstein cows, 5.1±0.8 years old (mean±s.e.m.), were given 10  ml saline day 10 (ovulation=day 1) a control estrous cycle. On subsequent cycle, they 0.5 μg/kg LPS. Luteal size decreased (from 5.2 3.8 cm(2), P≤0.05) within 24 h after LPS treatment remained smaller throughout remainder blood flow by 34% (P≤0.05) 3 lower for 72 h. Plasma progesterone (P(4)) concentrations increased first but subsequently declined. Following treatment, plasma prostaglandin (PG) F metabolites approximately tenfold higher in LPS-treated compared with cows (9.2 vs 0.8 ng/ml, 30 min, whereas PGE nearly double at 1 At 12 levels mRNA encoding Caspase-3 biopsies corpus luteum (CL) (P≤0.05), those StAR cattle saline. CASP3 protein localized cytoplasm and/or nuclei cells, detected cytosol cells. In cycle following either or LPS, there no significant differences between groups size, P(4) concentrations, gene expression. conclusion, diestrus transiently suppressed both function CL.