Comparative Molecular Modeling and Docking Analysis of β-galactosidase Enzymes from Commercially Important Starter Cultures Used in the Dairy Industry
作者： Vladimir Vukić , Dajana Hrnjez , Spasenija Milanović , Mirela Iličić , Katarina Kanurić
DOI: 10.1080/08905436.2015.1059766
关键词: Starter 、 Biology 、 Lactose 、 Docking (molecular) 、 Sequence alignment 、 Enzyme 、 Biochemistry 、 Molecular model 、 Beta-galactosidase 、 Hydrolysis
摘要: β-Galactosidases from S. thermophillus, L. acidophilus, and B. animalis lactis are essential enzymes which hydrolyze lactose during commercial yogurt cheese production. thermophillus β-galactosidase is active in the human digestive tract, improving digestion lactose-intolerant individuals. Because X-ray crystal structures have not been determined, molecular models of these β-galactosidases were created for comparative structural analysis docking against lactose. Modeling results validated using homologous E. coli circulans. The structure E.coli complex with was used as a control. Structure-based sequence alignment identified catalytically residues GLU458/GLU546 GLU148/GLU307 acidophilus GLU164/GLU324 ssp. lactis, predicts involved recognition. These provide fram...
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