Antarctic Rahnella inusitata: A Producer of Cold-Stable β-Galactosidase Enzymes

摘要: There has been a recent increase in the exploration of cold-active β-galactosidases, as it offers new alternatives for dairy industry, mainly response to current needs lactose-intolerant consumers. Since extremophilic microbial compounds might have unique physical and chemical properties, this research aimed study capacity Antarctic bacterial strains produce β-galactosidases. A screening revealed 81 out 304 with β-galactosidase activity. The strain Se8.10.12 showed highest enzymatic Morphological, biochemical, molecular characterization based on whole-genome sequencing confirmed first Rahnella inusitata isolate from Antarctic, which retained 41–62% its activity cold (4 °C–15 °C). Three β-galactosidases genes were found R. genome, belong glycoside hydrolase families GH2 (LacZ EbgA) GH42 (BglY). Based docking, some these enzymes exhibited higher lactose predicted affinity than commercial control enzyme Aspergillus oryzae. Hence, work reports continent prominent producer.