Highly sensitive electrochemical nuclear factor kappa B aptasensor based on target-induced dual-signal ratiometric and polymerase-assisted protein recycling amplification strategy.

摘要: In this work, an amplified electrochemical ratiometric aptasensor for nuclear factor kappa B (NF-κB) assay based on target binding-triggered signal readout and polymerase-assisted protein recycling amplification strategy is described. To demonstrate the effect of "signal-off" "signal-on" change dual-signal readout, thiol-hairpin DNA (SH-HD) hybridizes with methylene blue (MB)-modified protection (MB-PD) to form capture probes, which rationally introduced construction platform. On interface, probes can specifically bind NF-κB expose a toehold region subsequently ferrocene (Fc)-modified strand take Fc group electrode surface, accompanied by displacing MB-PD release MB from leading both (IFc) (IMB). order improve sensitivity aptasensor, phi29-assisted was designed achieve signal. With above advantages, prepared exhibits wide linear range 0.1pgmL-1 15ngmL-1 low detection limit 0.03pgmL-1. This provides simple ingenious approach construct shows promising potential applications in multiple disease marker changing recognition probe.