Determination of Transcription Nuclear Factor-Kappa B Using an Electrochemical, DNA-Based Nanoswitch

摘要: Traditional ways for the determination of transcription factor, which include Western blots, gel-shift assays, and quantitative polymerase chain reactions, are time-consuming complex. Here, a simple, electrochemical biosensor transcript nuclear factor kappa B (NF-κB) is reported. By incorporation NF-κB binding sequence hemin initiated DNA structure switch, dual functional probe that adopts stem-loop was immobilized on surface gold electrode. With NF-κB, protected from digestion by λ-exonuclease. The maintained nanostructure cannot capture hemins. No signal produced in this state. Without digested loop region liberated changed its to G-quadruplex with center, generating an signal. As result, sensitive ...