Glomerular C3c localization indicates ongoing immune deposit formation and complement activation in experimental glomerulonephritis.

摘要: In antibody-mediated glomerular disease, deposits of C3 (C3b) are common and degraded by factor I to C3c C3d. However, the kinetics C3b degradation in glomerulonephritis have not been defined. To do this, we studied three models complement-dependent with established (passive Heymann nephritis, cationized immunoglobulin G membranous nephropathy, concanavalin A-anticoncanavalin A glomerulonephritis). deposition was halted administration cobra venom factor, disappearance C3d from glomeruli measured specific antibodies quantitative fluorescence densitometry. Results showed that were reduced over 85% within 24 hours all models. clearance unaffected site or mechanism deposit formation. persisted despite lack ongoing complement activation. passive nephritis when disease activity monitored urinary C5b-9 excretion, cleared parallel return urine excretion normal values. We conclude cessation Positive staining for utilizing antibody portion documents recent activation usually reflecting new immune