Glomerular CR1 express in situ cofactor activity for degradation of C3b.

摘要: Adherence of sheep erythrocytes (E) sensitized with IgM antibodies (A) and C3b (EAC3b) to C3b/C4b receptors (CR1) in cryostat sections human renal glomeruli was studied using the closed chamber technique. The adsorption stable for at least 3 h 37 degrees C. In presence purified factor I, indicator cells, however, detached from after 30 min Factor H not required. release due loss CR1 activity tissue. cells were negative immune adherence test agglutinated by antibody C3d, but C3c. Western blot revealed C3d C3c found fluid. Accordingly, detachment degradation into Protease inhibitors did prevent cells. EAC3b incubated preincubated anti-CR1 degraded. results therefore indicate that situ can provide necessary cofactor I-mediated