Isolation of enzymatically homogeneous populations of human lymphocytes, monocytes, and granulocytes by zonal centrifugation.

摘要: Human monocytes, lymphocytes, granulocytes, red cells, and platelets were completely separated from each other by zonal centrifugation on linear sucrose density gradient. The monocytes contained only one tenth the amount of myeloperoxidase, half lysozyme, acid ,hosphatase, beta-glucuronidase found in granulocytes; no alkaline phosphatase or neutral protease. lymphocyte fraction amounts as much monocytes. Fluctuations enzyme levels granulocytes noted following infection. In vitro, isolated transformed into macrophages. results suggest that may be linked biochemically a differentiation sequence through sets commonly shared enzymes well groups specific for divergent cell line.