Genetic analysis of potato tuber quality traits

摘要: In this thesis the results of four year project P5 within Centre for BioSystems Genomics ( www.cbsg.nl ) are described. The intention was to gain understanding genetic principles underlying a series different potato tuber quality traits and development molecular markers linked interest. Molecular that can be used in breeding programs with aim producing varieties improved quality. To provide window which research placed, review current status genetics is given chapter 1. Here applicable described methodologies discussed. Enzymatic discoloration (ED) tubers investigated 2. QTL analysis trait itself an it’s potential substrates performed combination allele specificity study (including expression analysis) on candidate genes PPO. 3, starch related biosynthesis degradation by gene approach. Furthermore, 4 we analyzed metabolic content through liquid chromatography-time flight mass spectrometry (LC-QTOF MS). This resulted large set peaks. Clustering signals multivariate spectra reconstruction strategy revealed reconstructed metabolites (ea centrotypes). Quantitative Trait Locus (QTL) centrotypes over 800 metabolite QTLs (mQTLs), distributed all 12 chromosomes. investigate contribution metabolome phenotypic traits, mQTL were compared flesh colour (raw after-cooking) total protein content. Finally 5, general discussion, obtained preceding chapters discussed placed bigger perspective finding tools potato. Prospects basis these outlook presented as well.