Effects of repetitive gentle handling of male C57BL/6NCrl mice on comparative behavioural test results.

摘要: Mice are the most commonly used laboratory animals for studying diseases, behaviour, and pharmacology. Behavioural experiment battery aids in evaluating abnormal behaviour mice. During behavioural experiments, mice frequently experience human contact. However, effects of repeated handling on remains unclear. To minimise stress, methods moving using transparent tunnels or cups have been recommended but impractical tests. investigate these effects, we a test to assess differences between accustomed experimenter’s versus control Repeatedly handled gained slightly more weight than In tests, repeatedly showed improved spatial cognition Y-maze reduced anxiety-like elevated plus-maze test. there was no change light/dark transition open-field Grip strength, rotarod, sociability, tail suspension, Porsolt forced swim, passive avoidance tests revealed significant Our findings demonstrated that by experimenter before anxiety about high altitudes cognition, suggesting contact can affect results some