The AC133+CD38−, but not the rhodamine-low, phenotype tracks LTC-IC and SRC function in human cord blood ex vivo expansion cultures

摘要: Phenotypic markers associated with human hematopoietic stem cells (HSCs) were developed and validated using uncultured cells. Because phenotype function can be dissociated during culture, better to prospectively track isolate HSCs in ex vivo cultures could instrumental advancing HSC-based therapies. Using an expansion system previously shown increase progenitors SCID-repopulating (SRCs), we demonstrated that the rhodamine-low was lost, whereas AC133 expression retained throughout culture. Furthermore, AC133+CD38− subpopulation significantly enriched long-term culture-initiating (LTC-IC) SRCs after Preculture postculture analysis of total nucleated cell LTC-IC number, limiting dilution NOD/SCID mice, showed a 43-fold corresponded 7.3-fold 4.4-fold LTC-ICs this subpopulation, respectively. Thus, is improved marker tracks enriches for SRC cultures.