Low rhodamine 123 retention identifies long-term human hematopoietic stem cells within the Lin-CD34+CD38- population.
作者： Joby L. McKenzie , Katsuto Takenaka , Olga I. Gan , Monica Doedens , John E. Dick
DOI: 10.1182/BLOOD-2006-06-030270
关键词:
摘要: Progress to uncover the molecular and cellular regulators that govern human hematopoietic stem cell (HSC) fate has been impeded by an inability obtain highly purified fractions of HSCs. We report rhodamine 123 (Rho 123) dye effluxing fraction Lin-CD34+CD38- population contains SCID-repopulating cells (SRCs) capable long-term repopulation in primary nonobese diabetic/severe combined immunodeficient (NOD/SCID) mice. Purification based on Rho uptake led a 4-fold enrichment SRCs fraction, with frequency 1 SRC 30 Lin-CD34+CD38-Rholo cells. The also possesses self-renewal capacity as measured serial transplantation totaling more than 20 weeks. conclude efflux provides additional means purifying HSCs quest achieve homogeneous populations primitive for both experimental therapeutic applications.
ashpublications.org
bloodjournal.org
elsevier.com参考文章(19)
Bertoncello I, Hodgson Gs, Bradley Tr, Multiparameter analysis of transplantable hemopoietic stem cells: I. The separation and enrichment of stem cells homing to marrow and spleen on the basis of rhodamine-123 fluorescence. Experimental Hematology. ,vol. 13, pp. 999- 1006 ,(1985)
M Tsudo, T Tanaka, T Taniguchi, F Kitamura, H Karasuyama, M Miyasaka, M Hatakeyama, T Kono, A novel monoclonal antibody against murine IL-2 receptor beta-chain. Characterization of receptor expression in normal lymphoid cells and EL-4 cells. Journal of Immunology. ,vol. 147, pp. 2222- 2228 ,(1991)
MiJung Kim, Donna D. Cooper, Stanley F. Hayes, Gerald J. Spangrude, Rhodamine-123 staining in hematopoietic stem cells of young mice indicates mitochondrial activation rather than dye efflux. Blood. ,vol. 91, pp. 4106- 4117 ,(1998) , 10.1182/BLOOD.V91.11.4106
Gerard J. Madlambayan, Ian Rogers, Daniel C. Kirouac, Nobuko Yamanaka, Frèdèric Mazurier, Monica Doedens, Robert F. Casper, John E. Dick, Peter W. Zandstra, Dynamic changes in cellular and microenvironmental composition can be controlled to elicit in vitro human hematopoietic stem cell expansion. Experimental Hematology. ,vol. 33, pp. 1229- 1239 ,(2005) , 10.1016/J.EXPHEM.2005.05.018
L. V. Johnson, M. L. Walsh, L. B. Chen, Localization of mitochondria in living cells with rhodamine 123 Proceedings of the National Academy of Sciences of the United States of America. ,vol. 77, pp. 990- 994 ,(1980) , 10.1073/PNAS.77.2.990
Joby L. McKenzie, Olga I. Gan, Monica Doedens, John E. Dick, Human short-term repopulating stem cells are efficiently detected following intrafemoral transplantation into NOD/SCID recipients depleted of CD122+ cells. Blood. ,vol. 106, pp. 1259- 1261 ,(2005) , 10.1182/BLOOD-2005-03-1081
E. Conneally, J. Cashman, A. Petzer, C. Eaves, Expansion in vitro of transplantable human cord blood stem cells demonstrated using a quantitative assay of their lympho-myeloid repopulating activity in nonobese diabetic–scid/scid mice Proceedings of the National Academy of Sciences of the United States of America. ,vol. 94, pp. 9836- 9841 ,(1997) , 10.1073/PNAS.94.18.9836
Frédéric Mazurier, Monica Doedens, Olga I Gan, John E Dick, Rapid myeloerythroid repopulation after intrafemoral transplantation of NOD-SCID mice reveals a new class of human stem cells. Nature Medicine. ,vol. 9, pp. 959- 963 ,(2003) , 10.1038/NM886
Patricia Benveniste, Claude Cantin, Deborah Hyam, Norman N. Iscove, Hematopoietic stem cells engraft in mice with absolute efficiency. Nature Immunology. ,vol. 4, pp. 708- 713 ,(2003) , 10.1038/NI940
Frédéric Mazurier, Olga I. Gan, Joby L. McKenzie, Monica Doedens, John E. Dick, Lentivector-mediated clonal tracking reveals intrinsic heterogeneity in the human hematopoietic stem cell compartment and culture-induced stem cell impairment Blood. ,vol. 103, pp. 545- 552 ,(2004) , 10.1182/BLOOD-2003-05-1558