Novel proteomic approaches for tissue analysis
作者： Michael A Tangrea , Benjamin S Wallis , John W Gillespie , Gallya Gannot , Michael R Emmert-Buck
关键词: Western blot 、 Tissue proteomics 、 Molecular biology 、 Microdissection 、 Molecular analysis 、 Proteomics 、 Biology 、 Computational biology 、 2D Polyacrylamide Gel Electrophoresis 、 Protein expression 、 Biochemistry
摘要: Proteomics, the global study of protein expression and characteristics, has recently emerged as a key component in field molecular analysis. The dynamic nature proteins, from ion channels to chaperones, presents challenge, yet understanding these molecules provides rich source information. When applying proteomic analysis directly human tissue samples, additional difficulties arise. following article an overview current tools used tissues, beginning with conventional methods such western blot 2D polyacrylamide gel electrophoresis. most high-throughput techniques being today are also reviewed. These include arrays, reverse-phase lysate matrix-assisted laser desorption/ionization, surface-enhanced desorption/ionization layered scanning. In addition, bioinformatics well issues regarding preservation microdissection obtain pure cell popul...
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