Diterpenylhydroquinones from natural ent-labdanes induce apoptosis through decreased mitochondrial membrane potential.
作者： Joan Villena , Alejandro Madrid , Iván Montenegro , Enrique Werner , Mauricio Cuellar
DOI: 10.3390/MOLECULES18055348
关键词: Cell biology 、 Cytotoxicity 、 Apoptosis 、 Membrane potential 、 Cytotoxic T cell 、 Caspase 3 、 Cell culture 、 Biology 、 Cancer cell 、 Caspase
摘要: In this study, we examined the cytotoxic effects of seven ent-labdane derivatives 1-7 (0-100 μM) in different human cancer cell lines. Our results showed that compounds 1-3 exhibited significant dose-dependent inhibition on growth three lines, according to sulphorhodamine B assay and produced morphological changes consistent with apoptosis, as confirmed by Hoestch 3342 staining analysis. They induced apoptosis various shown nuclear condensation fragmentation caspase 3 activation. Such induction was associated depletion mitochondrial membrane potential. These activities led cleavage caspases trigger death process. Overall, potent proapoptotic two suggesting deserve more extensive investigation their potential medicinal applications.
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