Presynaptic Release of Glutamate by Heteroexchange Under Altered Gravity Conditions
作者： Tatiana Borisova , Natalia Krisanova
DOI: 10.1007/S12217-008-9049-9
关键词: Protonophore 、 Excitatory amino-acid transporter 、 Glutamate receptor 、 Synaptic vesicle 、 Cytosol 、 Hypergravity 、 Biophysics 、 Gravity (chemistry) 、 Chemistry 、 Metabotropic glutamate receptor
摘要: The study focused on the release of glutamate by reversal Na + -dependent transporters that was investigated in cortical synaptosomes isolated from Wistar rats subjected to centrifuge-induced hypergravity 10×g for 1 h. Transportable competitive inhibitor transporters—DL-threo-beta-hydroxyaspartate (DL-THA) induced L-[14C]glutamate via heteroexchange consisted 22.5 ± 1.7% total label normal gravity and 23.7 altered conditions. Inhibition ability synaptic vesicles accumulate neuromediator preliminary treatment with protonophore (FCCP) augmented cytosolic evoked DL-THA 44.0 2.0% 52.0 2.3% G-loaded animals as compared controls. Thus, combined application FCCP revealed increase transporter-mediated might be evidence hypoxic injury neurons.
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