Exposure of animals to artificial gravity conditions leads to the alteration of the glutamate release from rat cerebral hemispheres nerve terminals.

摘要: The biochemical basis underlying the effects of altered gravity on process nervous signal transmission is not clear. We have investigated effect hypergravity stress (created by centrifugation rats at l0 g for 1 h) basal and stimulated release L-[14C]glutamate (a chemical transmitter excitatory signals) from isolated rat brain nerve terminals (synaptosomes). It has been shown that exerted a different influence Ca(2+)-dependent Ca(2+)-independent component neurotransmitter release. evoked potassium chloride was equal to 14.4 +/- 0.7% total synaptosomal label control animals 6.2 1.9% animals, exposed (P < or = 0.05) more than twice decreased as result stress. observed no statistically significant difference in For group it 7.7 2.8% 12.9 2.0%, respectively. also activity high-affinity Na(+)-dependent glutamate transporters. Km Vmax uptake determined. maximal velocity loading, but values between observed. These findings indicate alters reuptake exocytotic processes.