Detection of Early Stage Apoptotic Cells Based on Label-Free Cytochrome c Assay Using Bioconjugated Metal Nanoclusters as Fluorescent Probes.
作者： Mojtaba Shamsipur , Fatemeh Molaabasi , Saman Hosseinkhani , Fereshteh Rahmati
DOI: 10.1021/ACS.ANALCHEM.5B03824
关键词: Nanoclusters 、 Chemistry 、 Förster resonance energy transfer 、 Molecular biology 、 DNA 、 Cytochrome c 、 Quenching (fluorescence) 、 Heme 、 Photoinduced electron transfer 、 Fluorescence 、 Biophysics
摘要: Cytochrome c (Cyt c) is an important biomarker in cell lysates for the early stage of apoptosis or anticancer agents. Here, two novel label-free fluorescence assays based on hemoglobin-stabilized gold nanoclusters (Hb/AuNCs) and aptamer-stabilized silver (DNA/AgNCs) analysis Cyt are presented. The heme group protein induces sensitive sensing platforms accompanied by decreased both metal nanoclusters. quenching processes observed found to be resonance energy transfer mechanism from Hb/AuNCs photoinduced electron DNA/AgNCs aptamer-Cyt complex. linear range was 0–10 μM 0 1 DNA/AgNCs, with limits detection ∼15 nM. On basis strong binding affinity DNA aptamers their target proteins, probe successfully applied quantitative determination lysates, which opens a new avenue d...
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