mTOR Pathway Activation Following Sciatic Stimulation in Wild-Type and Desmin Knockout Mice

摘要: mTOR Pathway Activation Following Sciatic Stimulation in Wild-Type and Desmin Knockout Mice Daniel S. Nelson Department of Exercise Sciences, BYU Master Science The 52 kDa intermediate filament protein desmin plays an important role force transmission skeletal muscle by connecting myofibrils at Z-lines to the sarcolemma. content adapts contractile activity may be involved cellular signaling mechanisms responsible for growth. Purpose: To compare responses pathway wild type (WT) vs knock out (KO) mice. Methods: WT (n=12) KO mice were exposed high frequency electric stimulation left hindlimb elicit acute response pathway. Non-stimulated right hindlimbs used as a within animal control. Right TA EDL muscles dissected 30 min post-stimulation examined changes mTOR, 4E-BP1 p70S6K. Results: Relative control samples, total was higher samples. Electrical resulted increase p70S6K phosphorylation animals however there no difference between groups. increased but not following electrical stimulation. There change or KO. Conclusion: absence does impair demonstrating that tensile load on will likely result synthesis. Elevated levels imply adaptation sensitivity growth stimuli muscle.