作者: Yoshiko Kawai , Toshio Ohhashi
关键词: Lymph node stromal cell 、 Lymphatic Endothelium 、 Endothelial stem cell 、 Sentinel lymph node 、 Lymph 、 Pathology 、 Immunohistochemistry 、 Podoplanin 、 Medicine 、 Lymphatic system
摘要: We examined the immunohistochemical properties of selective lymph vessel markers such as LYVE-1, podoplanin, Prox-1, and VEGF R3, well NO synthase (NOS) cyclo-oxygenase (COX) in two kinds human lymphatic endothelial cell isolated from collecting initial vessels. The constitutively expressed genes were also evaluated using oligonucleotide microarray analysis RT-PCR. investigated effects oxygen concentration culture conditions on proliferative activities cell. Immunoreactivity to LYVE-1 RT-PCR expression level mRNA cells vessels stronger than those ecNOS, iNOS, COX1, COX2 was found be significantly higher In contrast, an increase O2 ranging 5% 21% caused a significant reduction activity conclusion, these findings suggest that there exists marked heterogeneity immunohistochemical, genomic, between (*English Translation J Jpn Coll Angiol, 2008, 48: 125-130.)