Multiplex Cytological Profiling Assay to Measure Diverse Cellular States

摘要: Computational methods for image-based profiling are under active development, but their success hinges on assays that can capture a wide range of phenotypes. We have developed multiplex cytological assay “paints the cell” with as many fluorescent markers possible without compromising our ability to extract rich, quantitative profiles in high throughput. The detects seven major cellular components. In pilot screen bioactive compounds, detected phenotypes and it clustered compounds similar annotated protein targets or chemical structure based profiles. results demonstrate captures subtle patterns combination morphological labels, thereby detecting effects even though not stained directly. This provides an unbiased approach characterize compound- disease-associated cell states support future probe discovery.