Validated Reverse-Phase High-Performance Liquid Chromatography for Quantification of Furosemide in Tablets and Nanoparticles

摘要: A simple, sensitive, and specific method for furosemide (FUR) analysis by reverse-phase-HPLC was developed using a Spherisorb C18 ODS 2 column. chromatographic carried out mobile phase consisting of acetonitrile 10 mM potassium phosphate buffer solution: 70 : 30 (v/v) at pH 3.85, flow rate 1 mL·min−1. The UV-detection 233 nm room temperature. Validation parameters including limit detection (LOD), quantitation (LOQ), linearity range, precision, accuracy, robustness, specificity were investigated. Results indicated that the calibration curve linear () in range 5.2 to 25,000 ng·mL−1, with e value equal  L·M−1·cm−1. LOD LOQ found be 15.8 ng·mL−1, respectively. accurate (RSD less than 2%), precise, an intraday interday RSD 1.233–1.509 1.615 1.963%. stability native FUR has also been performed simulated perilymph endolymph media (with respective potency each medium % %, ) after 6 hours. This may routinely used quantitative from nanocarriers, USP tablets release related hearing research