Transmission FTIR derivative spectroscopy for estimation of furosemide in raw material and tablet dosage form.

摘要: A Fourier transform infrared derivative spectroscopy (FTIR-DS) method has been developed for determining furosemide (FUR) in pharmaceutical solid dosage form. The involves the extraction of FUR from tablets with N,N-dimethylformamide by sonication and direct measurement liquid phase mode using a reduced path length cell. In general, spectra were measured transmission equipment was configured to collect spectrum at 4 cm−1 resolution 13 s collection time (10 scans co-added). collected between 1400 cm−1 450 cm−1. Derivative used data processing quantitative peak area second order major spectral band found 1165 cm−1 (SO2 stretching FUR) baseline correction. fulfilled most validation requirements 2 mg/mL 20 mg/mL range, 0.9998 coefficient determination obtained simple calibration model, general variation <2%. mean recovery proposed assay resulted within (100±3)% over 80%–120% range target concentration. results agree pharmacopoeial and, therefore, could be considered interchangeable.