Efficient Adenovirus Gene Transfer Methods in Human Colonic Caco-2 Epithelial Cells Using Capric Acid

摘要: Adenovirus (Ad) vectors are widely used in gene therapy and vitro/in vivo transfer. However, Ad-mediated transfer epithelial cells shows low efficiency, because Ad fiber cannot bind to the primary receptor, coxsackievirus adenovirus receptor (CAR), present tight junctions. Caco-2 monolayer cultured on Transwell-chamber plates for approximately 2 weeks drug membrane permeation studies, but is difficult cells. First, we examined efficiency of into Luciferase production transduced with was 20-fold lower day 12 than 1. In contrast, expression CAR protein gradually increased along duration culture. For efficient cells, binding ability found be important. Capric acid (C10), a medium-chain fatty tight-junction modulator as pharmaceutical agent. We that novel method using transduction presence C10 led more efficiently LacZ alone. The results study indicate could very useful human colonic