Two-Photon Microperimetry: A Media Opacity-Independent Retinal Function Assay.

摘要: Purpose Compare results obtained using infrared two-photon microperimetry (2PM-IR) with conventional visual function tests in healthy subjects of varying ages and without simulated media opacities. Methods Subjects from two separate cohort studies completed cone contrast threshold (CCT) testing, microperimetry, visible light a novel device (2PM-Vis), microperimetry. The first study, which consisted six volunteers (23 to 29 years age), evaluated the effects opacities on performance testing. underwent testing four devices nine times under following conditions: no filter, red green blue brown dark polarized black filter (0° rotation), (90° rotation). subsequently performed 2PM-IR 2PM-Vis mydriatic state. second study effect age test 42 split between groups (ages 20-40 60-80 years). Results Retinal sensitivity measured by demonstrated lower variability than all other relying spectrum stimuli. decreased proportionally transmittance through each filter. CCT scores retinal modalities. Visible modalities larger result differences young old patient cohorts; this difference was inversely proportional wavelength test. Conclusions mitigates that may mask small when tested devices. Translational Relevance Conventional emit not detect during early stages age-related diseases due confounding cataracts. Infrared light, has greater ocular tissue, reliably quantify thereby degenerative changes on.