Simultaneous Flow Cytometric Quantification of Plant Nuclear DNA Contents Over the Full Range of Described Angiosperm 2C Values

摘要: Flow cytometry provides a rapid, accurate, and simple means to determine nuclear DNA contents (C-value) within plant homogenates. This parameter is extremely useful in number of applications basic applied biology; for example, it an important starting point projects involving whole genome sequencing, facilitates characterization species natural agricultural settings, allows facile identification engineered plants that are euploid or represent desired ploidy classes, points toward studies concerning the role C-value growth development response environment terms evolutionary fitness, and, uncovering new unexpected phenomena (for example endoreduplication), uncovers avenues scientific enquiry. Despite ease method, C-values have been determined only around 2% described angiosperm (flowering plant) species. Within this small subset, one most remarkable observations range 2C values, which spans at least two orders magnitude. In determining species, technical issues encountered relate both requirement method can provide accurate measurements across extended dynamic range, accommodate large amounts debris accompanies flow study, use Accuri C6 cytometer analysis described. work indicates unusually C6, design feature, coupled linearity fluorescence emission conferred by staining nuclei using propidium iodide, simultaneous whose span almost entire angiosperms. ' 2009 International Society Advancement Cytometry