Positive ‘atopy patch tests’ reactions in IgE‐hyperresponsive beagle dogs are dependent upon elevated allergen‐specific IgE serum levels and are associated with IgE‐expressing dendritic cells

摘要: In human patients with atopic dermatitis, the development of inflammation after epicutaneous application allergens is dependent upon presence IgE and Langerhans’ cells. The purpose this study was to investigate role allergen-specific in generation positive patch test reactions experimentally sensitized IgE-hyperresponsive beagle dogs. Patch tests were performed on seven dogs Dermatophagoides farinae (Df) flea saliva (FS) or saline control. Two had elevated serum against Df, one FS, two both not hypersensitive either allergen. Positive macroscopic consisted erythema, oedema induration, developed between 24 96 h allergen application. Macroscopic microscopic only when present tested allergens. Serum levels positively correlated dermal cell counts at 48 h (Spearman r = 0.59; P = 0.03) (r = 0.68; P < 0.01) challenge. Df-sensitized dogs, associated numerous IgE-staining epidermal dendritic aggregates. Dendritic cells for also expressed CD1c, identifying as These observations suggest that are IgE-dependent, lesions resemble those seen spontaneously-arising dermatitis. ‘Atopy Tests’ therefore could provide a good model investigating pathogenesis treatment skin canine species.  This supported, part, by Heska Corporation.