Activation of antigen-enriched b cells. I. Purification and response to thymus-independent antigens.

摘要: The development of a procedure for the selection large number functional antigen-binding B cells is described. Forty to 70% in enriched population bind antigen, and can be inhibited by pretreatment with either free hapten (TNP-Lys) or anti-immunoglobulin. express both slgM slgD respond mitogenic stimuli proliferating developing into antibody-forming cells. In contrast normal spleen cells, however, also proliferate response submitogenic levels TNP-Brucella abortus TNP-LPS.