Single Lipopolysaccharide-Reactive B Cells in the Non-Immune Mouse Spleen Cell Population Secrete Natural Multispecific Autoantibodies
作者： J. RAGIMBEAU , S. AVRAMEAS
DOI: 10.1111/J.1365-3083.1987.TB02231.X
关键词: Tubulin 、 Spleen 、 Population 、 Molecular biology 、 Actin 、 Precursor cell 、 Antibody 、 Splenocyte 、 Cell 、 Biology
摘要: Starting from unimmunized BALB/c splenocytes, B-cell clones were obtained by lipopolysaccharide (LPS) stimulation, and the frequencies of their anti-TNP, anti-BALB/c actin, tubulin secretion determined. The culture conditions then chosen so as to have one anti-TNP precursor per positive well. Out 41 wells containing antibody-secreting cell, nine (22%) also reacted either with actin or five (12%) both tubulin. Using horse red blood cells which trinitrophenyl (TNP) had been coupled, spleen cell rosettes prepared, enriched, micromanipulated, cultured individually. Of 500 micromanipulated TNP antigen-binding cells, 28 found secrete antibodies directed against TNP. Eight these (28%) tubulin, (17%) frequency multispecific is same order magnitude that limiting dilution experiments. results show mouse contains natural autoantibodies.
sci-hub.se 参考文章(25)
James A. Spudich, Susan Watt, The Regulation of Rabbit Skeletal Muscle Contraction Journal of Biological Chemistry. ,vol. 246, pp. 4866- 4871 ,(1971) , 10.1016/S0021-9258(18)62016-2
Ellen S Vitetta, E. C. Snow, J. W. Uhr, Activation of antigen-enriched b cells. I. Purification and response to thymus-independent antigens. Journal of Immunology. ,vol. 130, pp. 607- 613 ,(1983)
P Lymberi, A Coutinho, S Avrameas, G Dighiero, D Holmberg, I Lundquist, High frequency of natural autoantibodies in normal newborn mice. Journal of Immunology. ,vol. 134, pp. 765- 771 ,(1985)
P Lymberi, S Avrameas, G Dighiero, J C Mazié, G S Butler-Browne, R G Whalen, S Rouyre, Murine hybridomas secreting natural monoclonal antibodies reacting with self antigens. Journal of Immunology. ,vol. 131, pp. 2267- 2272 ,(1983)
Ellen S Vitetta, R. J. Noelle, K. G. Oliver, V. M. Sanders, E. C. Snow, J. W. Uhr, E. Yefenof, Preparation and analysis of antigen-specific memory B cells. Journal of Immunology. ,vol. 135, pp. 3777- 3784 ,(1985)
J.C. Cambier, M.J. Neale, Isolated phosphorylcholine binding lymphocytes. I. Use of a cleavable crosslinking reagent for solid-phase adsorbent isolation of functional antigen binding cells. Journal of Immunological Methods. ,vol. 51, pp. 209- 221 ,(1982) , 10.1016/0022-1759(82)90260-5
J. Russell Little, Herman N. Eisen, Preparation and characterization of antibodies specific for the 2,4,6-trinitrophenyl group. Biochemistry. ,vol. 5, pp. 3385- 3395 ,(1966) , 10.1021/BI00875A001
B. Michael Longenecker, Tim R. Mosmann, “Natural” Antibodies to chicken MHC antigens are present in mice, rats, humans, alligators and allogeneic chickens Immunogenetics. ,vol. 11, pp. 293- 302 ,(1980) , 10.1007/BF01567795
THéRESE TERNYNCK, STRATIS AVRAMEAS, Polymerization and Immobilization of Proteins Using Ethylchloroformate and Glutaraldehyde Scandinavian Journal of Immunology. ,vol. 5, pp. 29- 35 ,(1976) , 10.1111/J.1365-3083.1976.TB03834.X
M. L. Shelanski, F. Gaskin, C. R. Cantor, Microtubule Assembly in the Absence of Added Nucleotides Proceedings of the National Academy of Sciences of the United States of America. ,vol. 70, pp. 765- 768 ,(1973) , 10.1073/PNAS.70.3.765