DNA hypomethylation and proliferative activity are increased in the rectal mucosa of patients with long-standing ulcerative colitis.
作者： Luísa Glória , Marília Cravo , António Pinto , L. Salazar de Sousa , Paula Chaves
DOI: 10.1002/(SICI)1097-0142(19961201)78:11<2300::AID-CNCR5>3.0.CO;2-Q
关键词: Intestinal mucosa 、 Proliferative index 、 Methyltransferase 、 DNA hypomethylation 、 DNA methylation 、 Immunology 、 Cancer research 、 Medicine 、 Cell cycle 、 Epigenetics 、 Methylation
摘要: BACKGROUND DNA methylation and DNA cytometric parameters were evaluated in the rectal mucosa from patients with extensive long-standing ulcerative colitis. METHODS Twenty-six disease for more than 7 years 11 healthy controls included. Global was assessed as capacity of test to incorporate [3H]methyl groups [3H]-S-adenosylmethionine presence Sss1 methylase. A higher incorporation reflects a lower state intrinsic methylation. ploidy, S-phase fraction, proliferative index (PI = S + G2M) cell cycle analyzed by flow cytometry. RESULTS Incorporation into 10-fold compared (P 0.05). CONCLUSIONS DNA hypomethylation activity are increased this group patients, supporting concept that their colonic undergoes epigenetic kinetic changes might predispose these individuals develop colorectal neoplasms. However, it cannot be ruled out markers solely reflect hyperproliferation associated active inflammation. Cancer 1996;78:2300-6.
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