K-ras mutations and p53 alterations in neoplastic and nonneoplastic lesions associated with longstanding ulcerative colitis.

摘要: We have analyzed K-ras mutations and p53 alterations in 39 tumor nontumor samples taken from nine patients with longstanding ulcerative colitis colorectal carcinoma. Two of invasive carcinomas contained a mutation. By combination immunohistochemistry single-strand conformation polymorphism analysis, were found three carcinomas. Five 13 dysplastic lesions harbored mutated gene, even the absence detectable changes associated tumors. One single focus mucosa concomitant gene alterations. In two patients, mutation was detected epithelial considered to be devoid malignant potential (villous regeneration, active colitis). Our results indicate that: 1) prevalence genetic colitis-associated colonic appears lower than sporadic carcinomas; 2) can dysplasia, villous affect subpopulation cells composing lesions; 3) diverse same patient exhibit different genotype 4) at least part regeneration should as preneoplastic colitis.