Infectious bronchitis virus poly-epitope-based vaccine protects chickens from acute infection
作者： Lei Tan , Yuqiang Zhang , Fang Liu , Yanmei Yuan , Yuan Zhan
DOI: 10.1016/J.VACCINE.2016.09.022
关键词: DNA vaccination 、 Epitope 、 Neutralization 、 Cellular immunity 、 Infectious bronchitis virus 、 Immune system 、 Virology 、 Viral shedding 、 Immunization 、 Microbiology 、 Biology
摘要: Infectious bronchitis virus (IBV) causes major losses in the poultry industry. The safe and effective vaccine to control IBV spread is imperative. In present study, we developed S1 glycoprotein poly-epitope-based DNA pV-S1B+S1T consisting of SH1208 Holte strain BF2-restricted T cell epitopes Australian dominant B neutralization epitopes. Specific pathogen-free chickens were vaccinated with plasmids twice elicit strong humoral cellular immune response, as indicated by viral titers results CD8+ proliferation assays. A lethal dose used for protection challenge experiments at two weeks post-booster immunization following shedding reverse transcription quantitative PCR assay, that protected against significantly reduced excretion. These demonstrated effectively prevents infection represents a potential vaccine.
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