Molecular epidemiology of enterovirus B77 isolated from non polio acute flaccid paralytic patients in Pakistan during 2013

摘要: Abstract Human enteroviruses are associated with various clinical syndromes and severe neurological disorders. The aim of this study was to determine the molecular epidemiology non polio their correlation acute flaccid paralysis (AFP) patients living in Khyber Pakhtunkhwa (KP) Federally Administered Tribal Areas (FATA) Pakistan. stool samples collected from these were used for isolation (NPEVs). Out 38 samples, 29 (76.3%) successfully typed by microneutralization assay into eleven serotypes including echovirus (E)-3 (5.3%), E-7 (2.6%), E-11 (13.2%), E-12 (7.9%), E-13 (10.5%), E-20 E-27 E-29 E-30 E-33 coxsackievirus (CV) B5 (2.6%) nine isolates (23.7%) remained untyped which confirmed as NPEVs real time RT-PCR. Complete VP1 genetic sequencing data characterized untypeable enterovirus B77 (EV-B77). Moreover, phylogenetic analysis classified viruses two new genotypes having high diversity (at least 17.7%) prototype. This provides valuable information on extensive EV-B77 genotypes. Although, its association disorder has not yet been known but AFP cases highlights fact that they may have a contributing role etiology AFP. In addition, it is needed establish surveillance system laboratory diagnostic facilities early detection cause poliomyelitis like especially situation when we at verge eradication.