Inhibition of Sphingolipid Synthesis by Cycloserine In Vitro and In Vivo
作者： K. Soma Sundaram , Meir Lev
DOI: 10.1111/J.1471-4159.1984.TB02716.X
关键词: Cycloserine 、 Biochemistry 、 Enzyme assay 、 Sphingolipid 、 Enzyme 、 In vivo 、 Biology 、 Cerebroside 、 Ganglioside 、 In vitro 、 Cellular and Molecular Neuroscience
摘要: : d- and l-cycloserine were shown to be irreversible inhibitors of the first enzyme sphingolipid pathway, 3-ketodihydrosphingosine synthetase, in a study using bacterial brain microsomal enzymes, l-Cycloserine was 100 times more inhibitory than d-isomer for vitro. In vivo, caused 70% inhibition enzyme. Following one injection, activity recovered 80% normal after 16 hours. Daily dosages on regimen intraper-itoneal injection 7 days significant reduction total ganglioside cerebroside plus sulfatide levels.
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