Enzymatic activities on cell walls in bacteriophage T4

摘要: Abstract When isolated particles of bacteriophage T4 are disrupted by freezing and thawing, they exhibit a lytic activity (particle-bound lysozyme). T4eam phage, which has an amber mutation in the structural gene for phage lysozyme, propagated restrictive host is devoid this activity. In addition, temperature-sensitive mutant lysozyme exhibits particle-bound also temperature sensitive. It follows from these facts that found free lysate directed same gene, e. The not essential to infection nor ‘lysis-from-without’, although events were postulated be enzymatic. An enzymatic attacks cell walls Escherichia coli different was phage. differs following respects. (1) optimum pH enzyme lies between 3.5 5, while around 7. (2) lyses neither chloroform-treated cells intact Micrococcus lysodeikticus. (3) deficient lysozyme.