The synthesis of messenger RNA without protein synthesis

摘要: A polyauxotrophic mutant of Escherichia coli which is deficient in the ability to synthesize thymine, uracil and histidine has been used analyse virus-induced processes making specific nucleic acids enzymes. The RNA made this T6 r + -infected strain absence required amino acid or presence chloramphenicol characteristic base ratios electrophoretic mobility normal phage-induced RNA, mainly associated with ribosomes. Incubation isolated RNA-charged ribosomes inorganic phosphate leads selective degradation RNA. This infected cells degraded actively either histidine. In histidine, products then appear DNA. Thus, phage-infected produce protein synthesis. deoxycytidylate hydroxymethylase, thymidylate synthetase lysozyme that order at different times after infection. syntheses these enzymes are completely inhibited appearance considerably delayed uracil, never attaining a high level. After charging synthesis, addition essential produces stimulated rate synthesis early enzymes, hydroxymethylase synthetase. However, such prior does not significantly affect nor even time appearance, late enzyme, lysozyme. From results we suggest determines proteins alone, sequential production phage determined by On infection inactivated u.v. light, continues for considerable time, whereas DNA strongly inhibited. Our consistent hypothesis cessation inception require