Plant regeneration from protoplasts of Japanese lawngrass.
作者： Chie Inokuma , Kiyoyuki Sugiura , Chol Cho , Ryoji Okawara , Seiji Kaneko
DOI: 10.1007/BF00232218
关键词: Botany 、 Regeneration (biology) 、 Biology 、 Plantlet 、 Murashige and Skoog medium 、 Poaceae 、 Cell culture 、 Callus 、 Zoysia japonica 、 Protoplast
摘要: Embryogenic callus of Japanese lawngrass (Zoysia japonica Steud.) was induced from sterile mature seeds on LS medium with 5 mg / l 2,4-D. selected visually under microscope proliferated in liquid N6 amino acids (N6-AA medium). Protoplasts were isolated suspension cells by the treatment enzyme mixture containing pectolyase Y-23 and cultured K8p 2 2,4-D at density 106 ml. Plants regenerated transferring protoplasts derived to MS incubating 28 °C light for two months. Plantlets successfully transplanted soil.
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