금잔디 포복경으로부터 식물체 재분화에 있어서 식물생장조절물질과 배지첨가물질의 영향
作者： 이병현 , 허성현 , 배은지 , 김용구 , 이광수
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摘要: To optimize tissue culture conditions for genetic transformation of Zoysia matrella (L.) Merr., we investigated the effects different plant growth regulators and medium supplements on regeneration using stolon explants excised from mature grown in a green house. Plant frequency was 33.3% when tissues with node were cultured supplemented 0.5 mg/L 2,4-D 1 kinetin. Comparing basal media tested, MS showed higher performance than N6 or SH medium. Addition 5 AgNO 3 10 cysteine improved up to 40%. Among carbon sources, 3% sucrose found show best frequency. This rapid efficient system would be useful experiments manilagrass without intervening callus-mediated regeneration.
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