Effect of Plant Growth Regulators and Antioxidants on Callus Induction and Plant Regeneration from Seed Culture of Orchardgrass
作者：
DOI: 10.5333/KGFS.2005.25.3.191
关键词:
摘要: In order to optimize tissue culture conditions for genetic transformation of orchardgrass, the effects medium supplements on responses were investigated with mature seeds 3 cultivars, `Frode`. `Roughrider` and `Frontier` as explant tissues. Callus induction containing 3mg/L 2,4-D or dicamba each 0.1mg/L BA was optimal fer embryogenic callus formation from seed had a strong effect successive plant regeneration. The regeneration frequency among cultivars descending Roughrider > Frode Frontier. Supplementation media 10mg/L 40mg/L cysteine enhanced Efficient system established in this study will be useful fur molecular breeding orchardgrass through transformation.
koreascience.or.kr参考文章(13)
Gil A. Enríquez-Obregón, Dmitri L. Prieto-Samsónov, Gustavo A. de la Riva, Marlene Pérez, Guillermo Selman-Housein, Roberto I. Vázquez-Padrón, Agrobacterium-mediated Japonica rice transformation: a procedure assisted by an antinecrotic treatment Plant Cell Tissue and Organ Culture. ,vol. 59, pp. 159- 168 ,(1999) , 10.1023/A:1006307527449
Vikrant, A. Rashid, Somatic embryogenesis from immature and mature embryos of a minor millet Paspalum scrobiculatum L. Plant Cell Tissue and Organ Culture. ,vol. 69, pp. 71- 77 ,(2002) , 10.1023/A:1015048529095
Susan Eapen, Leela George, Plant regeneration from peduncle segments of oil seed Brassica species: Influence of silver nitrate and silver thiosulfate Plant Cell Tissue and Organ Culture. ,vol. 51, pp. 229- 232 ,(1997) , 10.1023/A:1005926108586
Ashok Chaudhury, Rongda Qu, Somatic embryogenesis and plant regeneration of turf-type bermudagrass: Effect of 6-benzyladenine in callus induction medium Plant Cell Tissue and Organ Culture. ,vol. 60, pp. 113- 120 ,(2000) , 10.1023/A:1006456005961
R. N. Trigiano, D. J. Gray, B. V. Conger, J. K. McDaniel, Origin of Direct Somatic Embryos from Cultured Leaf Segments of Dactylis glomerata Botanical Gazette. ,vol. 150, pp. 72- 77 ,(1989) , 10.1086/337750
P. van der Valk, F. Ruis, A. M. Tettelaar-Schrier, C. M. van de Velde, Optimizing plant regeneration from seed-derived callus cultures of Kentucky bluegrass. The effect of benzyladenine Plant Cell Tissue and Organ Culture. ,vol. 40, pp. 101- 103 ,(1995) , 10.1007/BF00041125
B. V. CONGER, G. E. HANNING, D. J. GRAY, J. K. MCDANIEL, Direct Embryogenesis from Mesophyll Cells of Orchardgrass Science. ,vol. 221, pp. 850- 851 ,(1983) , 10.1126/SCIENCE.221.4613.850
Toshio Murashige, Folke Skoog, A Revised Medium for Rapid Growth and Bio Assays with Tobacco Tissue Cultures Physiologia Plantarum. ,vol. 15, pp. 473- 497 ,(1962) , 10.1111/J.1399-3054.1962.TB08052.X
M. E. Horn, R. D. Shillito, B. V. Conger, C. T. Harms, Transgenic plants of Orchardgrass (Dactylis glomerata L.) from protoplasts Plant Cell Reports. ,vol. 7, pp. 469- 472 ,(1988) , 10.1007/BF00272733
Myeong-Je Cho, Wen Jiang, Peggy G. Lemaux, Transformation of recalcitrant barley cultivars through improvement of regenerability and decreased albinism Plant Science. ,vol. 138, pp. 229- 244 ,(1998) , 10.1016/S0168-9452(98)00162-9