A Simple Method for Measuring Carbon-13 Fatty Acid Enrichment in the Major Lipid Classes of Microalgae Using GC-MS

摘要: A simple method for tracing carbon fixation and lipid synthesis in microalgae was developed using a combination of solid-phase extraction (SPE) negative ion chemical ionisation gas chromatography mass spectrometry (NCI-GC-MS). NCI-GC-MS is an extremely sensitive technique that can produce unfragmented molecular making this particularly useful stable isotope enrichment studies. Derivatisation fatty acids pentafluorobenzyl bromide (PFBBr) allows the coupling high separation efficiency GC measurement ions each by single quadrupole MS. The key spectra be measured without interference from co-eluting or other molecules. Pre-fractionation extracts SPE 13C incorporation into three main classes (phospholipids, glycolipids, neutral lipids) thus allowing study complex biochemistry relatively straightforward analytical technology. selectivity necessary as it collection individual acids, including cis/trans isomers, PFB-derivatised acids. GC-MS enables accurate determination pool. Three solvent protocols are commonly used lipidomics were also evaluated described here with regard to efficiencies analysis microalgae.