Two Distinct Monooxygenases for Alkane Oxidation in Nocardioides sp. Strain CF8

摘要: Alkane monooxygenases in Nocardioides sp. strain CF8 were examined at the physiological and genetic levels. Strain can utilize alkanes ranging chain length from C2 to C16. Butane degradation by butane-grown cells was strongly inhibited allylthiourea, a copper-selective chelator, while hexane-, octane-, decane-grown showed detectable butane activity presence of allylthiourea. Growth on hexane 1-hexyne, 1-hexyne did not affect growth octane or decane. A specific 30-kDa acetylene-binding polypeptide observed for butane-, but absent grown with decane 1-hexyne. These results suggest two CF8. Degenerate primers designed PCR amplification genes related binuclear-iron-containing alkane hydroxylase Pseudomonas oleovorans used clone gene Reverse transcription-PCR Northern blot analysis that this encoding expressed above C6. indicate distinct oxidation