Molecular Mechanisms of Enhanced Bacterial Growth on Hexadecane with Red Clay.

摘要: Red clay was previously used to enhance bioremediation of diesel-contaminated soil. It speculated that the enhanced degradation diesel due increased bacterial growth. In this study, we selected Acinetobacter oleivorans DR1, a soil-borne degrader and alkanes, as model bacterium performed transcriptional analysis using RNA sequencing investigate cellular response during hexadecane utilization mechanism by which red promotes degradation. We confirmed growth A. DR1 on hexadecane, major component diesel, sole carbon source. Addition hexadecane-utilizing cells highly upregulated β-oxidation, while genes related alkane oxidation were expressed with without clay. also oxidative stress defense, such superoxide dismutase, catalase, glutaredoxin genes, suggesting supports generated utilization. Increased membrane fluidity in presence fatty acid methyl ester at different phases, could be uptake coupled upregulation downstream metabolism defense. The monitoring community soil for year revealed stabilized structure.