Natural montmorillonite as support for the immobilization of inulinase from Kluyveromyces marxianus NRRL Y-7571
作者： Chaline C. Coghetto , Robison P. Scherer , Marceli F. Silva , Simone Golunski , Sibele B.C. Pergher
DOI: 10.1016/J.BCAB.2012.06.005
关键词: Kluyveromyces marxianus 、 Sucrose 、 Enzyme 、 Biochemistry 、 Specific activity 、 Montmorillonite 、 Inulin 、 Inulinase 、 Nuclear chemistry 、 Chemistry
摘要: Abstract The objective of this study was to investigate the process immobilization inulinases using natural montmorillonite as inorganic support. enzyme buffer ratio 3:10 and 10 min led highest specific activity, 375.07 U/mg protein. immobilized inulinase kept its activity after 1968 h under storage at low temperatures 456–1826 h high temperatures. pH value 3.5 activity. Km values 1.46 0.38 mM, vmax 0.2487 0.2396 mol/L min, were obtained, respectively, for sucrose inulin.
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