Fructose production from inulin using fungal inulinase immobilized on 3-aminopropyl-triethoxysilane functionalized multiwalled carbon nanotubes
作者： Ram Sarup Singh , Kanika Chauhan , John F. Kennedy
DOI: 10.1016/J.IJBIOMAC.2018.11.281
关键词:
摘要: Abstract The main objective of the present work was to modify multiwalled carbon nanotubes (MWCNTs) using 3-aminopropyl-triethoxysilane (APTES) generate amino-terminated surfaces for inulinase immobilization, which can be further used fructose production. CCRD response surface methodology optimization immobilization on MWCNTs. At optimized parameters (APTES concentration 4%; sonication time 4 h; enzyme coupling 1.5 h and load 15 IU), maximal activity yield 60.7% 74.4%, respectively. Immobilized showed same pH optima free enzyme, while an elevation in temperature 60 °C observed after its immobilization. also shown enhancement stability thermostability. Overall, 4.54-fold rise half-life detected at 60 °C. Km Vmax decreased preserved 28% residual 10 consecutive batch cycles inulin hydrolysis production fructose.
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