Design and characterisation of an enzyme system for inulin hydrolysis

摘要: Abstract The optimal conditions for inulin hydrolysis using a commercial inulinase preparation, either free or immobilised in activated Amberlite were established by factorial design and surface response methodology. biocatalyst displayed highest activity at pH 5.5 50 °C, whereas the optimum form was slightly more acidic (4.5), temperature little higher (55 °C). model system estimated values of 5.4 52 °C 4.9 56 °C system. Michaelis–Menten type kinetics adequately described both bioconversion systems, which evaluated under respective conditions. use non-linear regression method determination kinetic parameters provided best fit to experimental data, as compared conventional Lineweaver–Burk linearisation. K m 153 g l −1 55 °C 4.5, apparent 108 g l 50 °C. reutilisation throughout consecutive batches evaluated. A significant decrease enzyme observed first two batches, after exhibited stability. low cost support, stability towards its high affinity substrate suggests potential hydrolysis.