Antioxidant Role of PcGSTd1 in Fenpropathrin Resistant Population of the Citrus Red Mite, Panonychus citri (McGregor).
作者： Chong-Yu Liao , Ying-Cai Feng , Gang Li , Xiao-Min Shen , Shi-Huo Liu
关键词: Mutant 、 Biology 、 Genetics 、 Gene 、 Lipid peroxidation 、 Antioxidant 、 Candidate gene 、 Gene expression 、 Acaricide 、 Population
摘要: The citrus red mite, Panonychus citri, a major pest distributed worldwide, has evolved severe resistance to various classes of chemical acaricides/insecticides including pyrethroids. It is well known that the pyrethroids mainly caused by point mutations voltage-gated sodium channel gene in wide range pests. However, increasing number evidences support might also be resulted from integrated mechanisms metabolic mechanisms. In this study, firstly, comparative analysis RNA-seq data showed multiple detoxification genes, GSTs PcGSTd1, were up-regulated fenpropathrin-resistant population compared with susceptible strain (SS). Quantitative real time-PCR results exposure fenpropathrin had an induction effect on transcription PcGSTd1 time-dependent manner. vitro inhibition and assay recombinant found not metabolized directly protein. its antioxidant role alleviating oxidative stress was demonstrated via reversely genetic experiment. Our provide list candidate genes which may contribute implicated evolution field P. citri. Furthermore, during process, plays detoxifying lipid peroxidation products induced fenpropathrin.
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Sharda P. Singh, Julia A. Coronella, Helen Beneš, Bruce J. Cochrane, Piotr Zimniak, Catalytic function of Drosophila melanogaster glutathione S-transferase DmGSTS1-1 (GST-2) in conjugation of lipid peroxidation end products. FEBS Journal. ,vol. 268, pp. 2912- 2923 ,(2001) , 10.1046/J.1432-1327.2001.02179.X
William H. Habig, Michael J. Pabst, William B. Jakoby, Glutathione S-Transferases Journal of Biological Chemistry. ,vol. 249, pp. 7130- 7139 ,(1974) , 10.1016/S0021-9258(19)42083-8
Tian-Bo Ding, Rui Zhong, Xuan-Zhao Jiang, Chong-Yu Liao, Wen-Kai Xia, Bin Liu, Wei Dou, Jin-Jun Wang, Molecular characterisation of a sodium channel gene and identification of a Phe1538 to Ile mutation in citrus red mite, Panonychus citri Pest Management Science. ,vol. 71, pp. 266- 277 ,(2015) , 10.1002/PS.3802
PIE MÜLLER, MOUHAMADOU CHOUAÏBOU, PATRICIA PIGNATELLI, JOSIANE ETANG, EDWARD D. WALKER, MARTIN J. DONNELLY, FRÉDÉRIC SIMARD, HILARY RANSON, Pyrethroid tolerance is associated with elevated expression of antioxidants and agricultural practice in Anopheles arabiensis sampled from an area of cotton fields in Northern Cameroon. Molecular Ecology. ,vol. 17, pp. 1145- 1155 ,(2007) , 10.1111/J.1365-294X.2007.03617.X
Vassiliki Bariami, Christopher M. Jones, Rodolphe Poupardin, John Vontas, Hilary Ranson, Gene Amplification, ABC Transporters and Cytochrome P450s: Unraveling the Molecular Basis of Pyrethroid Resistance in the Dengue Vector, Aedes aegypti PLOS Neglected Tropical Diseases. ,vol. 6, ,(2012) , 10.1371/JOURNAL.PNTD.0001692
Nongkran Lumjuan, Shavanthi Rajatileka, Donch Changsom, Jureeporn Wicheer, Posri Leelapat, La-aied Prapanthadara, Pradya Somboon, Gareth Lycett, Hilary Ranson, The role of the Aedes aegypti Epsilon glutathione transferases in conferring resistance to DDT and pyrethroid insecticides Insect Biochemistry and Molecular Biology. ,vol. 41, pp. 203- 209 ,(2011) , 10.1016/J.IBMB.2010.12.005
He Lin, Xue Chuan-hua, Wang Jin-jun, Li Ming, Lu Wen-cai, Zhao Zhi-mo, Resistance selection and biochemical mechanism of resistance to two Acaricides in Tetranychus cinnabarinus (Boiduval) Pesticide Biochemistry and Physiology. ,vol. 93, pp. 47- 52 ,(2009) , 10.1016/J.PESTBP.2008.11.001
Guohua Qin, Miao Jia, Ting Liu, Xueyao Zhang, Yaping Guo, Kun Yan Zhu, Enbo Ma, Jianzhen Zhang, Characterization and Functional Analysis of Four Glutathione S-Transferases from the Migratory Locust, Locusta migratoria PLOS ONE. ,vol. 8, ,(2013) , 10.1371/JOURNAL.PONE.0058410
Jinfeng Hu, Changfang Wang, Jun Wang, Yong You, Feng Chen, Monitoring of resistance to spirodiclofen and five other acaricides in Panonychus citri collected from Chinese citrus orchards Pest Management Science. ,vol. 66, pp. 1025- 1030 ,(2010) , 10.1002/PS.1978
A. A. Enayati, H. Ranson, J. Hemingway, Insect glutathione transferases and insecticide resistance. Insect Molecular Biology. ,vol. 14, pp. 3- 8 ,(2005) , 10.1111/J.1365-2583.2004.00529.X