Molecular characterisation of a sodium channel gene and identification of a Phe1538 to Ile mutation in citrus red mite, Panonychus citri
作者： Tian-Bo Ding , Rui Zhong , Xuan-Zhao Jiang , Chong-Yu Liao , Wen-Kai Xia
DOI: 10.1002/PS.3802
关键词:
摘要: BACKGROUND The citrus red mite, Panonychus citri (McGregor), is regarded as one of the most serious pests in many countries and has developed high resistance to pyrethroids a result intensive use these acaricides. RESULTS The para sodium channel gene P. (named PcNav), containing an entire coding region 6729 bp, was cloned this study. Three alternative splicing sites 12 potential RNA editing were identified PcNav. Thus, exons alt 1 3-v3 found be unique Comparison field fenpropathrin-resistant (WZ) susceptible (LS) strains point mutation F1538I IIIS6 channel, which known confer strong mites. Moreover, it also that PcNav mRNA present during all life stages, transcript seems more abundant larvae than other developmental stages. CONCLUSION These results suggest plays important role fenpropathrin This first study provides information for further research on mechanism pyrethroid resistance. © 2014 Society Chemical Industry
sci-hub.se 参考文章(58)
Barry Ganetzky, Christopher J. Hanrahan, Robert A. Reenan, Michael J. Palladino, RNA editing of the Drosophila para Na(+) channel transcript. Evolutionary conservation and developmental regulation. Genetics. ,vol. 155, pp. 1149- 1160 ,(2000) , 10.1093/GENETICS/155.3.1149
JR Thackeray, B Ganetzky, DEVELOPMENTALLY REGULATED ALTERNATIVE SPLICING GENERATES A COMPLEX ARRAY OF DROSOPHILA PARA SODIUM CHANNEL ISOFORMS The Journal of Neuroscience. ,vol. 14, pp. 2569- 2578 ,(1994) , 10.1523/JNEUROSCI.14-05-02569.1994
J-Z. Niu, W. Dou, T-B. Ding, G-M. Shen, K. Zhang, G. Smagghe, J-J. Wang, Transcriptome analysis of the citrus red mite, Panonychus citri, and its gene expression by exposure to insecticide/acaricide. Insect Molecular Biology. ,vol. 21, pp. 422- 436 ,(2012) , 10.1111/J.1365-2583.2012.01148.X
Andrias O. O'Reilly, Bhupinder P. S. Khambay, Martin S. Williamson, Linda M. Field, B. A. WAllace, T. G. Emyr Davies, Modelling insecticide-binding sites in the voltage-gated sodium channel. Biochemical Journal. ,vol. 396, pp. 255- 263 ,(2006) , 10.1042/BJ20051925
Z. Liu, W. Song, K. Dong, Persistent tetrodotoxin-sensitive sodium current resulting from U-to-C RNA editing of an insect sodium channel Proceedings of the National Academy of Sciences of the United States of America. ,vol. 101, pp. 11862- 11867 ,(2004) , 10.1073/PNAS.0307695101
François Jeanmougin, Julie D. Thompson, Manolo Gouy, Desmond G. Higgins, Toby J. Gibson, Multiple sequence alignment with Clustal X Trends in Biochemical Sciences. ,vol. 23, pp. 403- 405 ,(1998) , 10.1016/S0968-0004(98)01285-7
Eddo Kim, Alon Magen, Gil Ast, Different levels of alternative splicing among eukaryotes Nucleic Acids Research. ,vol. 35, pp. 125- 131 ,(2007) , 10.1093/NAR/GKL924
N.N. Jonsson, C. Cutullè, S.W. Corley, J.M. Seddon, Identification of a mutation in the para-sodium channel gene of the cattle tick Rhipicephalus microplus associated with resistance to flumethrin but not to cypermethrin. International Journal for Parasitology. ,vol. 40, pp. 1659- 1664 ,(2010) , 10.1016/J.IJPARA.2010.07.006
Ruiwu Wang, Zachary Y. Huang, Ke Dong, Molecular characterization of an arachnid sodium channel gene from the varroa mite (Varroa destructor). Insect Biochemistry and Molecular Biology. ,vol. 33, pp. 733- 739 ,(2003) , 10.1016/S0965-1748(03)00068-7
Ming-Long Yuan, Dan-Dan Wei, Kun Zhang, Yu-Zhen Gao, Yong-Hua Liu, Bao-Jun Wang, Jin-Jun Wang, Genetic Diversity and Population Structure of Panonychus citri (Acari: Tetranychidae), in China Based on Mitochondrial COI Gene Sequences Journal of Economic Entomology. ,vol. 103, pp. 2204- 2213 ,(2010) , 10.1603/EC09392