Persistent tetrodotoxin-sensitive sodium current resulting from U-to-C RNA editing of an insect sodium channel

摘要: The persistent tetrodotoxin (TTX)-sensitive sodium current, detected in neurons of many regions mammalian brains, is associated with essential neuronal activities, including boosting excitatory synaptic inputs, acceleration firing rates, and promotion oscillatory activities. However, the origin molecular basis current have remained controversial for decades. Here, we provide direct evidence that U-to-C RNA editing an insect channel transcript generates a current. We TTX-sensitive splice variant cockroach gene BgNav (formerly paraCSMA). Site-directed mutagenesis experiments revealed F-to-S change at C-terminal domain this was responsible demonstrated result event, which also occurred Drosophila para transcript. Our work provides support hypothesis posttranscriptional modification conventional transient produces channel.