Molecular characterization of an arachnid sodium channel gene from the varroa mite (Varroa destructor).

摘要: Voltage-gated sodium channels are essential for the generation and propagation of action potentials in most excitable cells. They target sites several classes insecticides acaricides. Isolation full-length channel cDNA is a critical often difficult step toward an understanding insecticide acaricide resistance. We previously cloned sequenced two overlapping clones covering segment 3 domain II (IIS3) to 6 IV (IVS6) arachnid gene (named VmNa) from varroa mite (Varroa destructor) (J. Apicultureal Res. 40 (2002) 5.). In this study, we isolated three more revealed entire coding region VmNa (Genbank accession number: AY259834), thus providing first complete sequence gene. The composite contains 6645 nucleotides with open reading frame encoding 2215 amino acids. deduced acid shares 51% overall identity Drosophila Para 41% mammalian alpha-subunit Na(v)1.2. All hallmarks proteins conserved protein. Three optional exons one retained intron were identified VmNa. precise position size only exon insect genes Na(v)1.6 human, mouse fish, whereas other novel. Interestingly, novel located C-terminus, where no alternative have been any