In vivo and ex vivo tissue applications of two-photon microscopy.

摘要: Publisher Summary This chapter describes in vivo and ex tissue applications of two-photon microscopy (TPM) reviews key areas—such as neurobiology—in which TPM has already made a major impact. is one the emerging optical imaging techniques, it constructive to compare with other techniques. powerful technique for because its inherent 3D resolution long penetration depth. also provides biochemical information about tissues causes minimal photodamage. based on nonlinear fluorescence excitation process. The simultaneous absorption two infrared photons promotes transition fluorophore excited state. Because process, there only sufficient photon density very near focal region produce appreciable excitation, results an sectioning effect. unique advantages deep have been recognized nearly since inception. are proliferating rapidly.