Callus induction and shoot organogenesis from anther cultures of Curcuma attenuata Wall

摘要: Curcuma attenuata is a highly valued ornamental. This study provides the first report on C. shoot organogenesis and plant regeneration. Immature anthers derived from 5 to 7 cm long inflorescences were isolated cultured different variations of Murashige Skoog (MS) media induce callus then organogenesis. When 2-mm in which microspores at uninucleate developmental stage dark MS medium containing 13.6 μM 2,4-dichlorophenoxyacetic acid (2,4-D) 2.3 kinetin (KT) for 15 days transferred 40 μmol m−2 s−1 fluorescent light 30 days, percentage induction reached 33.3 %. After was various differentiation light, 33.1 % all cultures could differentiate into adventitious shoots supplemented with 22.0 6-benzyladenine (BA), 0.53 α-naphthaleneacetic (NAA) 1.4 thidiazuron (TDZ) after culturing 60 days. Over 95 plantlets survived transplanting trays mixture sand perlite (2: 1) 20 Chromosome number, determined root tips young plantlets, indicated that diploid (2n = 84).